Fig. 14. Effect of pre-treatment of WM266-4 cells with autophagy inhibitors on viability and levels of LC3-II and lamin A/C cleavage in the presence of 2155-14. (A) Representative western blot of lamin A/C. (B) Representative western blot of LC3. (C) Quantification of western blot of lamin A/C. 2155-14 was tested at 100 µM, LY and hydroxychloroquine (HCQ) were tested at 10 µM. (D) Quantification of western blot of LC3. The data shown are the mean ± SD, n=3. (E) Viability of WM266-4 cells after pre-treatment with autophagy inibitors in the presence of 2155-14. The data shown were the mean ± SD, n=6. One-way analysis of variance (ANOVA) was used followed by Dunnett post hoc test. ***** - p<0.0001, *** - p<0.001, ** - p<0.01, * - p<0.05, ns - no significance.